Phytochemical Estimation and Antioxidant Potential of Cinchona officinalis L. Stem Bark Extracts
DOI:
https://doi.org/10.22270/ijmspr.v9i2.65Keywords:
Cinchona officinalis L, Rubiaceae, Antioxidant activity, DPPH assay methodAbstract
Stem bark of Cinchona sp. (Rubiaceae) is one of the well known drugs for its therapeutic values in traditional as well as modern medicine. Even though a lot of work has been carried out on quinoline alkaloids of Cinchona, its phenolic constituents received very little attention. The aim of the present study was to evaluate in vitro antioxidant activities, qualitative and quantitative phytochemical analysis of Cinchona officinalis L (C. officinalis) stem bark collected from Bhopal region of Madhya Pradesh. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolic content were determined by the well-known test protocol available in the literature. The in vitro antioxidant activity of aqueous extract of the stem bark was assessed against DPPH radical scavenging assay methods using standard protocols. Phytochemical analysis revealed the presence of, carbohydrates, flavonoids, diterpines, saponins, protein and phenols. The total phenolic content of aqueous stem bark extract of C. officinalis was found to be 0.548mg/100mg respectively. The activities of aqueous extracts against DPPH assay method were concentration dependent. The diverse array of phytochemicals present in the plant thus suggests its therapeutic potentials which may be explored in drug manufacturing industry as well as in traditional medicine.
Keywords: Cinchona officinalis L, Rubiaceae, Antioxidant activity, DPPH assay method.
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