Performance Evaluation of Truenat rtPCR assay with COBAS -6800 in SARS COV-2 detection: A diagnostic accuracy study
DOI:
https://doi.org/10.22270/ijmspr.v11i3.157Keywords:
cobas 6800, TrueNet, COVID 19, Point of care testAbstract
Context: Diagnostic testing plays a critical role in early and accurate detection of SARS-COV-2. Real-time PCR is the Gold standard test for the diagnosis of SARS-COV-2 which requires well equipped BSL-2 Lab along with skilled manpower. There is a need for a rapid point of care test for diagnosis. TrueNat is one such testing system that is approved by Govt. of India.
Aims: To find out the performance of the TrueNat assay in comparison to cobas 6800.
Settings and Design: The Cross-sectional study was conducted in a COVID 19 Testing Laboratory in Central India.
Methods and Material: A total of 122 COVID-19 positive samples in cobas 6800 were analysed in TrueNat assay.
Results: Of the total of cobas positive122 sample TrueNat system were able to detect 116 samples with the viral load of high(n=18), medium(n=28 ), low(n=44),very low(n=26). Considering cobas 6800 as a Gold standard assay TrueNat test showed a sensitivity and specificity of 95% and 100%respectively.
Conclusions: Based on our findings we believe that TrueNat assay is equally effective for the diagnosis of SARS-COV-2 compared to a highly sophisticated instrument such as cobas 6800. Hence, it should be installed in all healthcare setups with a limited setting for rapid and reliable diagnosis of SARS-COV-2.
Keywords: cobas 6800, TrueNat, COVID-19, Point of care test.
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Copyright (c) 2025 Arindam Chakraborty , Monica Singh , Amod Kumar
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