INFLUENCE OF SNAKE NAJA NAJA VENOM ON DNA DAMAGE IN ALBINO RAT

M Malleswari; P Josthna; Dossp Jacob

doi:10.22270/ijmspr.v2i2.14

Authors

M Malleswari Department of Zoology, S V University, Department of Biotechnology, S P Mahila University, Tirupati-517502, AP.
P Josthna Department of Zoology, S V University, Department of Biotechnology, S P Mahila University, Tirupati-517502, AP.
Dossp Jacob Department of Zoology, S V University, Department of Biotechnology, S P Mahila University, Tirupati-517502, AP

DOI:

https://doi.org/10.22270/ijmspr.v2i2.14

Keywords:

Snake Naja naja venom, DNA damage, Agarose gel electrophoresis

Abstract

Snake venom has therapeutic effect on treatment of certain diseases. This study has carried out to determine the snake Naja naja venom on DNA fragmentation levels. An increase in DNA levels was observed and the electrophoretic studies revealed that there is a significant change in the DNA banding pattern and the banding pattern is species specific which plays an important role in species identification and taxonomy. The electrophoretic DNA patterns are consistent in 24 h, 48 h, 72 h of envenomated rats. The present study may be for the first time concentrated on DNA banding pattern in the envenomated rats and this banding pattern will have the significance in building up the different snake species in getting together under one umbrella of taxonomy

Author Biographies

M Malleswari , Department of Zoology, S V University, Department of Biotechnology, S P Mahila University, Tirupati-517502, AP.

Department of Zoology, S V University, Department of Biotechnology, S P Mahila University, Tirupati-517502, AP.

P Josthna , Department of Zoology, S V University, Department of Biotechnology, S P Mahila University, Tirupati-517502, AP.

Department of Zoology, S V University, Department of Biotechnology, S P Mahila University, Tirupati-517502, AP.

Dossp Jacob , Department of Zoology, S V University, Department of Biotechnology, S P Mahila University, Tirupati-517502, AP

Department of Zoology, S V University, Department of Biotechnology, S P Mahila University, Tirupati-517502, AP

References

Asselta R and Peyvandi F (2009), Factor V deficiency SeminThrombHemost., Vol. 35, No. 4, pp. 382-9.

Bahman Maroufi, Kaboudanian Sussan Ardestani, Amina Kariminia, Hussain Naderimanesh (2005) “The effect of vitamin E on splenocytes apoptosis of gamma irradiated BALB/C mice”, Iranian Journal of Allergy , Asthma and Immunology., Vol. 35, No. 1, pp. 77-82.

Bailey P Wilce (2001), “J Venom as a source of useful biologically active molecules”, Emergency Medicine, Vol. 13, pp. 28-36.

Brachet J and Jeener R (1944), “Macromolecular Cytoplasmic Particles Rich in Pentose Nucleic Acid. I. General properties Relation to Hydrolase, Hormones and Structural Proteins”, Enzymologia., Vol. 1, pp. 196-212.

Gillo L (1966), “Biochemistry of snake venom”, Toxicon. Ann. Soc Roy Sci Med Nat Bruxelles, Vol. 19, pp. 121.

Gosselin L, Bacq C M, Gosselin F C, Rey C, Kozma S and Osterieth P M (1977), “Phospholipids of the Milk-fat-globule Membrane and the Mouse Mammary Tumor virus isolated from the milk of infected mice”, Biochem. Soc. Trans., Vol. 5, pp. 142-144.

Hantgan P (2005), “Snake venom reveals clues about heart drug”, Baptist Medical., Vol. 1, pp. 1-3.

Holley R W (1968), “Biochemistry of snake venom”, Toxicon. Progr.Nucleic Acids Res, Vol. 8, pp. 37.

Horio T, Miyauchi-Hashimoto H, Kuwamoto K, Yamazaki F and Okamoto H (2007), “Photobiological information obtained from XPA gene-deficient mice”, Photochem Photobiol, Vol. 83, pp. 218–24.

Iwaguchi T, Hayashi K and Sakurai Y (1973), “Cytocidal Activity of Cytotoxin from Indian cobra venom & its derivatives against experimental tumours”, Cancer inst, George K Daikos (Ed.), Vol. III, Proceedings of the 8th International Congress of Chemotherapy Athens, Athens, pp. 755-758.

Nelson M, Lopera-Barrero, Jayme Povh A, Ricardo P, Ribeiro, Patricia C.Gomes, Carolina B, Jacometo and Tais da Silva Lopes (2008), Cien. Inv. Agr., Vol. 35, No. 1, pp. 65-74.

Shaikh D M (1985), “Effect of snake venom on cancerous cells and other proteins”, Ann. Rept. PSF, Lahore, Pakistan.

Shaikh D M, Jamali A G, Ansari A F and Seehar G M (1986), “Safe and effective dosage for normal (rabbit) and human hepatic cancer cells”, Pak J Zoo., Vol. 18, pp. 187-93.

Sunita Kumari, Rajesh P, Rastogi, Kanchan L, Singh Shailendra P, Singh, Rajeshwar P Sinha (2008), EXCLI Journal, Vol. 7, pp. 44- 62 , ISSN 1611-2156